Research on the Repeated Use of Novel Ferrocene-Tagged Nanomaterial for Determination of Glucose

Abstract

A good novel double ferrocene-tagged nanomaterial is designed for glucose determination and its recycling stability. Double Ferrocene-tagged nanomaterial displays higher catalytic activity toward the glucose oxidation than non-ferrocene-tagged nanomaterial. In this study novel nanoparticles including double ferrocene, N-{2-[Bis(2-aminoethyl)amino]ethyl}aminomethyl-polystyrene (2AEPS) and ferrocene aldehyde (Fc) have been synthesized by means of condensation and investigated the enzymatic properties of glucose oxidase enzyme (GOD) immobilized on there. Double ferrocene-tagged nanomaterial-GOD shows high reusability and storage capacity and fast incubation time determination of glucose. 2AEPSFc-GOD retains more than 15% of the initial activity after forty five successive cycles, which is a perfect performance.