dc.contributor.author | Sarı, Nursen | |
dc.contributor.author | Antepli, Esin | |
dc.contributor.author | Nartop, Dilek | |
dc.contributor.author | Yetim, Nurdan Kurnaz | |
dc.date.accessioned | 2021-12-12T17:02:03Z | |
dc.date.available | 2021-12-12T17:02:03Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 1661-6596 | |
dc.identifier.uri | https://doi.org/10.3390/ijms130911870 | |
dc.identifier.uri | https://hdl.handle.net/20.500.11857/3367 | |
dc.description.abstract | Modified polystyrene with Pt(IV)-azomethine (APS-Sch-Pt) was synthesized by means of condensation and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on it. The characteristics of the immobilized glucose oxidase (APS-Sch-Pt-GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7. The insertion of stable Pt(IV)-azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS-Sch-Pt-GOx), increases the enzymes' activity and improves their affinity towards the substrate even at pH = 4. The influence of temperature, reusability and storage capacity on the free and immobilized glucose oxidase enzyme was investigated. The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 degrees C. | en_US |
dc.description.sponsorship | Gazi University Research FundGazi University [05/2010-75, 05/2012-68] | en_US |
dc.description.sponsorship | This work was supported by the Gazi University Research Fund (Project number: 05/2010-75 and 05/2012-68). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Mdpi Ag | en_US |
dc.relation.ispartof | International Journal of Molecular Sciences | en_US |
dc.identifier.doi | 10.3390/ijms130911870 | |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Pt(IV)-azomethine | en_US |
dc.subject | Polystyrene | en_US |
dc.subject | glucose oxidase enzyme | en_US |
dc.subject | two optimum pH | en_US |
dc.title | Polystyrene Attached Pt(IV)-Azomethine, Synthesis and Immobilization of Glucose Oxidase Enzyme | en_US |
dc.type | article | |
dc.department | Fakülteler, Fen-Edebiyat Fakültesi, Kimya Bölümü | |
dc.identifier.volume | 13 | en_US |
dc.identifier.startpage | 11870 | en_US |
dc.identifier.issue | 9 | en_US |
dc.identifier.endpage | 11880 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.authorscopusid | 7003536574 | |
dc.authorscopusid | 55371252100 | |
dc.authorscopusid | 25230320600 | |
dc.authorscopusid | 55934629400 | |
dc.identifier.wos | WOS:000309272700074 | en_US |
dc.identifier.scopus | 2-s2.0-84866896048 | en_US |
dc.identifier.pmid | PubMed: 23109888 | en_US |
dc.authorwosid | SARI, NURSEN/AAA-8328-2021 | |