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dc.contributor.authorAsav, Engin
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2021-12-12T17:00:45Z
dc.date.available2021-12-12T17:00:45Z
dc.date.issued2014
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2014.02.032
dc.identifier.urihttps://hdl.handle.net/20.500.11857/2886
dc.description.abstractA specific and sensitive biosensor was developed successfully for quantitative detection of human epidermal growth factor receptor by electrochemical impedance spectroscopy. Anti-human epidermal growth factor receptor antibody was covalently immobilized onto a screen-printed carbon electrode modified with a carbon nanotube. Immobilization steps were characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). After human epidermal growth factor receptor ligates with anti-human epidermal growth factor receptor immobilized onto an electrode surface, charge transfer resistance changes considerably. This electrochemical response was correlated with human epidermal growth factor receptor concentration. Under optimal conditions, the proposed biosensor could detect human epidermal growth factor receptor 2 fg/mL with a linear range from 2 to 14 fg/mL, showing high sensitivity. Kramers-Kronig Transform was performed on the experimental impedance data. Meanwhile, in a biosensor system, the Single Frequency Impedance technique was first used for characterization of interaction between human epidermal growth factor receptor and anti-human epidermal growth factor receptor. Eventually, the proposed biosensor was applied to artificial serum samples spiked with human epidermal growth factor receptor. (C) 2014 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [109T 172]en_US
dc.description.sponsorshipSupport from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 109T 172) is greatly acknowledged. Also we are grateful to Namik Kemal University Scientific and Technological Research Center (NABILTEM).en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.identifier.doi10.1016/j.ijbiomac.2014.02.032
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCancer biomarkersen_US
dc.subjectBiosensoren_US
dc.subjectHuman epidermal growth factor receptoren_US
dc.subjectElectrochemical impedance spectroscopyen_US
dc.subjectSingle frequency impedanceen_US
dc.titleA novel impedimetric disposable immunosensor for rapid detection of a potential cancer biomarkeren_US
dc.typearticle
dc.authoridAsav, Engin/0000-0002-6232-3388
dc.departmentFakülteler, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümü
dc.identifier.volume66en_US
dc.identifier.startpage273en_US
dc.identifier.endpage280en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.authorscopusid25935968900
dc.authorscopusid6506764918
dc.identifier.wosWOS:000335201900036en_US
dc.identifier.scopus2-s2.0-84900354655en_US
dc.identifier.pmidPubMed: 24560951en_US
dc.authorwosidAsav, Engin/AAG-1170-2021
dc.authorwosidAsav, Engin/AAG-4182-2021
dc.authorwosidSezginturk, Mustafa Kemal/AAY-1439-2021


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