Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms

Abstract

New polymeric microspheres containing azomethine (1a-1c and 2a-2c) were synthesized by condensation to compare the enzymatic properties of the enzyme glucose oxidase (GOx) and to investigate antimutagenic and antimicrobial activities. The polymeric microspheres were characterized by elemental analysis, infrared spectra (FT-IR), proton nuclear magnetic resonance spectra, thermal gravimetric analysis, and scanning electron microscopy analysis. The catalytic activity of the glucose oxidase enzyme follows Michaelis-Menten kinetics. Influence of temperature, reusability, and storage capacity of the free and immobilized glucose oxidase enzyme were investigated. It is determined that immobilized enzymes exhibit good storage stability and reusability. After immobilization of GOx in polymeric supports, the thermal stability of the enzyme increased and the maximum reaction rate (V-max) decreased. The activity of the immobilized enzymes was preserved even after 5 months. The antibacterial and antifungal activity of the polymeric microspheres were evaluated by well-diffusion method against some selected pathogenic microorganisms. The antimutagenic properties of all compounds were also examined against sodium azide in human lymphocyte cells by micronuclei and sister chromatid exchange tests. Novel polymeric microspheres including azomethine with Pt(IV) were synthesized by means of condensation method. Polymeric microspheres were characterized by means of spectral measurements. Glucose oxidase (GOx) enzyme was covalently immobilized on these polymeric microspheres and investigated the enzymatic properties of GOx. The catalytic activity of GOx enzyme followed Michaelis-Menten kinetics. The antibacterial and antifungal activities of all polymeric microspheres were investigated by the well-diffusion method as antimicrobial agents. The antimutagenic properties of these polymeric microspheres were evaluated against sodium azide (NaN3) in human lymphocyte cells by micronuclei (MN) and sister chromatid exchange (SCE) tests.